Electrochemical Enzyme Immunoassay Using Sequential Saturation Technique in A 20-μl Capillary: Digoxin as A Model Analyte

Authors

Noriaki Kaneki, University of Cincinnati
Yan Xu, Cleveland State UniversityFollow
Anu Kumari, University of Cincinnati
H. Brian Halsall, University of Cincinnati
William R. Heineman, University of Cincinnati
Peter T. Kissinger, Purdue University

Document Type

Article

Publication Date

3-21-1994

Publication Title

Analytica Chimica Acta

Abstract

Capillary enzyme immunoassay with flow-injection analysis for digoxin using the sequential saturation technique has been developed. Glass capillary tubes (10 cm × 0.53 mm i.d.) with immobilized digoxin antibody were used as the immunoassay reactor. The product of enzymatic reaction. 4-aminophenol, was detected amperometrically. The digoxin and the labeled digoxin binding reaction with the immobilized digoxin antibody were completed in 2 and 10 min, respectively. Digoxin was determined in a 20-μl sample with a detection limit of 10 pg ml−1 (200 fg or 260 attomoles) and a 3 orders of magnitude range.

Recommended Citation

Kaneki, Noriaki; Xu, Yan; Kumari, Anu; Halsall, H. Brian; Heineman, William R.; and Kissinger, Peter T., "Electrochemical Enzyme Immunoassay Using Sequential Saturation Technique in A 20-μl Capillary: Digoxin as A Model Analyte" (1994). Chemistry Faculty Publications. 187.
https://engagedscholarship.csuohio.edu/scichem_facpub/187

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

DOI

10.1016/0003-2670(93)E0612-B

Version

Postprint

Volume

287

Issue

3