Title

Rapid Analysis of Docetaxel in Human Plasma by Tandem Mass Spectrometry with On-line Sample Extraction

Document Type

Article

Publication Date

2004

Publication Title

Journal of Pharmaceutical and Biomedical Analysis

Abstract

A simple, rapid, and sensitive analytical method for the measurement of docetaxel in human plasma was developed and validated. The method is based on positive electrospray ionization tandem mass spectrometry (ESI+–MS–MS) with on-line sample extraction. It uses paclitaxel as internal standard for calibration. The on-line sample extraction minimizes sample handling and is readily adopted for automation. Quantitation of plasma docetaxel was done by the multiple reaction monitoring (MRM) mode. The method had a linear calibration range of 1.00–3000ng/mL with a correlation coefficient >0.9999. The limit of quantitation (LOQ) for docetaxel in plasma was 1.00ng/mL. The on-line extraction recovery of docetaxel was between 86.1–94.7%, with %CV ≤ 6.1%. This method has high accuracy (90.1–96.3%), and excellent intra-assay (0.6–3.8%) and inter-assay (2.0–5.7%) precision. Its applicability to clinical samples was demonstrated by measuring patient plasma samples after treatment of weekly docetaxel at 25mg/m2 as 60-min infusion.

DOI

10.1016/j.jpba.2004.05.014

Volume

36

Issue

1