Selective Determination of A Group of Organic Compounds in Complex Sample Matrixes by LC/MIMS with On-Line Immunoaffinity Extraction
Document Type
Article
Publication Date
3-1-1998
Publication Title
Analytical Chemistry
Abstract
An on-line immunoaffinity extraction with liquid chromatography/membrane introduction mass spectrometry (IAE/LC/MIMS) method for the determination of BTEX compounds in complex sample matrixes is described. This method uses an immunoaffinity column (1 mm i.d. × 20 mm) for on-line sample cleanup and enrichment, a 5-μm C18 trapping column (2 mm i.d. × 20 mm) for analyte focusing, a 3-μm C18 analytical column (3.2 mm i.d. × 100 mm) for separation, and a membrane introduction mass spectrometer for quantitation. The immunoaffinity column was evaluated in terms of binding capacity, recovery, and enrichment factor. The method was optimized for the determination of BTEX compounds in a mixture of 30 volatile organic compounds, which showed no matrix interference and a dramatic improvement of the detection limit over that of the LC/MIMS method (up to 474-fold). This method was also used for the determination of BTEX compounds in several gasoline-contaminated water samples, and the results were compared with the EPA reference methods.
Recommended Citation
Ouyang, Shi; Xu, Yan; and Chen, Yong Hong, "Selective Determination of A Group of Organic Compounds in Complex Sample Matrixes by LC/MIMS with On-Line Immunoaffinity Extraction" (1998). Chemistry Faculty Publications. 276.
https://engagedscholarship.csuohio.edu/scichem_facpub/276
DOI
10.1021/ac971039y
Volume
70
Issue
5
