Title

Selective Determination of A Group of Organic Compounds in Complex Sample Matrixes by LC/MIMS with On-Line Immunoaffinity Extraction

Document Type

Article

Publication Date

3-1-1998

Publication Title

Analytical Chemistry

Abstract

An on-line immunoaffinity extraction with liquid chromatography/membrane introduction mass spectrometry (IAE/LC/MIMS) method for the determination of BTEX compounds in complex sample matrixes is described. This method uses an immunoaffinity column (1 mm i.d. × 20 mm) for on-line sample cleanup and enrichment, a 5-μm C18 trapping column (2 mm i.d. × 20 mm) for analyte focusing, a 3-μm C18 analytical column (3.2 mm i.d. × 100 mm) for separation, and a membrane introduction mass spectrometer for quantitation. The immunoaffinity column was evaluated in terms of binding capacity, recovery, and enrichment factor. The method was optimized for the determination of BTEX compounds in a mixture of 30 volatile organic compounds, which showed no matrix interference and a dramatic improvement of the detection limit over that of the LC/MIMS method (up to 474-fold). This method was also used for the determination of BTEX compounds in several gasoline-contaminated water samples, and the results were compared with the EPA reference methods.

DOI

10.1021/ac971039y

Volume

70

Issue

5