A Simple and Quantitative Method for Analysis of Methoxyamine by Capillary Zone Electrophoresis
Journal of Liquid Chromatography & Related Technologies
Methoxyamine (MX) is a potential new chemotherapeutic agent. In structure, MX lacks a chromophore for spectrophotometric detection. Derivatization of MX with chromophoric 4‐(diethylamino)benzaldehyde (DEAB) was performed and the resultant MX derivative (DBMOH + ) can be analyzed by capillary zone electrophoresis (CZE) with ultraviolet (UV) detection at 200 nm. The limit of detection (SD/S=3.3) of the method for MX was 2.50 µM with a 2 s injection at the pressure of 3.45 kPa. The linear calibration range for MX was 5.00–500 µM using N,N ‐dimethyl‐ p ‐toluidine (DMPT) as the internal standard. The intra‐ and inter‐assay precision and accuracy of the method were ≤4% and within 98.4–104%, respectively. This method may be used for the quantitative determination of MX in pharmaceutical preparations.
Yu-Chieh Liao; Mei-Jywan Syu; Xu, Y. A Simple and Quantitative Method for Analysis of Methoxyamine by Capillary Zone Electrophoresis. J. Liq. Chromatogr. Rel. Technol. 2005, 28, 2433-2443.