Title

Intracellular Nitric Oxide Measurement in Human Sperm Using 4, 5-DiaminoFluorescein-2-Diacetate and Flow Cytometry

Document Type

Poster

Publication Date

9-1-2011

Publication Title

Fertility and Sterility

Abstract

Objective: Nitric oxide (NO) has a major contribution in male reproduction, especially in sperm motility. Methods for direct measurement of NO in single cell such as spermatozoa are complex, lack sensitivity, and are non-specific. The objective of this study was to standardize the measurement of intracellular nitric oxide in human spermatozoa using NO specific probe 4,5-diaminofluorescein-2-diacetate (DAF-2DA) and flow cytometry.

DOI

10.1016/j.fertnstert.2011.07.892

Volume

96

Issue

3