Document Type
Article
Publication Date
2015
Publication Title
Analytical & Bioanalytical Techniques
Abstract
BMCL26 is a potential drug derived from nimesulide, which has exhibited the substantial anti-parasitic activity in various cell lines. To conduct various pharmacological and toxicological properties of this drug, we developed and validated a rapid LC-MS/MS method for its quantification in accordance with the FDA guidelines. Protein precipitation with 0.1% formic acid in acetonitrile was used to extract the analytes along with the internal standard (JCC76) from rat plasma. It was found that the calibration curve of the method had an excellent linearity (r2 ≥ 0.9993) for the analyte concentration ranging from 0.5 to 100 ng/mL with acceptable inter- and intra-assay, precision, accuracy and stability. The matrix effect and extraction recovery were in the range of 101.30-110.10% and 90.16- 105.00%, respectively. This LC-MS/MS method is simple and rapid and can be used in the future pharmaceutical studies of BMCL26.
Recommended Citation
Voggu, Ramakrishna R.; Zhou, Xiang; Su, Bing; and Guo, Baochuan, "A Simple and Rapid LC-MS/MS Method for The Determination of BMCL26 A Novel Anti-Parasitic Agent in Rat Plasma" (2015). Chemistry Faculty Publications. 377.
https://engagedscholarship.csuohio.edu/scichem_facpub/377
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
DOI
10.4172/2155-9872.1000266
Version
Publisher's PDF
Volume
6
Issue
5
Comments
The authors acknowledge the following sources of support for this work: NIH grant R15AI 103889 (B. Su) and the National Science Foundation Major Research Instrumentation Grant (CHE-0923398).