Document Type

Article

Publication Date

2015

Publication Title

Analytical & Bioanalytical Techniques

Abstract

BMCL26 is a potential drug derived from nimesulide, which has exhibited the substantial anti-parasitic activity in various cell lines. To conduct various pharmacological and toxicological properties of this drug, we developed and validated a rapid LC-MS/MS method for its quantification in accordance with the FDA guidelines. Protein precipitation with 0.1% formic acid in acetonitrile was used to extract the analytes along with the internal standard (JCC76) from rat plasma. It was found that the calibration curve of the method had an excellent linearity (r2 ≥ 0.9993) for the analyte concentration ranging from 0.5 to 100 ng/mL with acceptable inter- and intra-assay, precision, accuracy and stability. The matrix effect and extraction recovery were in the range of 101.30-110.10% and 90.16- 105.00%, respectively. This LC-MS/MS method is simple and rapid and can be used in the future pharmaceutical studies of BMCL26.

Comments

The authors acknowledge the following sources of support for this work: NIH grant R15AI 103889 (B. Su) and the National Science Foundation Major Research Instrumentation Grant (CHE-0923398).

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

DOI

10.4172/2155-9872.1000266

Version

Publisher's PDF

Volume

6

Issue

5

Included in

Chemistry Commons

Share

COinS