Interference by Naturally Occurring Fatty Acids in A Noncellular Enzyme-Based Aromatase Bioassay
Document Type
Article
Publication Date
4-1-2006
Publication Title
Journal of Natural Products
Abstract
Natural product drug discovery efforts frequently utilize noncellular screening assays. Fatty acids are commonly found in natural product extracts, and some have been shown to interfere with noncellular assays. Several pure fatty acids were tested using a noncellular aromatase assay, with the unsaturated analogues showing strong inhibitory activity, while the saturated analogues were inactive. Unsaturated fatty acids were further tested against SK-BR-3 hormone-independent human breast cancer cells that overexpress aromatase and were found to be inactive. In natural product screening efforts, especially using plant seeds, it is recommended that extracts active in noncellular bioassays should be dereplicated for the presence of fatty acids prior to bioassay-guided fractionation.
Recommended Citation
Balunas, Marcy J.; Su, Bin; Landini, Serena; Brueggemeier, Robert W.; and Kinghorn, A. Douglas, "Interference by Naturally Occurring Fatty Acids in A Noncellular Enzyme-Based Aromatase Bioassay" (2006). Chemistry Faculty Publications. 434.
https://engagedscholarship.csuohio.edu/scichem_facpub/434
DOI
10.1021/np050513p
Volume
69
Issue
4
Comments
This research was supported by a University Fellowship from the University of Illinois at Chicago (to M.J.B.), NIH grant R01 CA73698 (P.I., R.W.B.), The Ohio State University Comprehensive Cancer Center (OSUCCC) Breast Cancer Research Fund (to R.W.B.), and the OSUCCC Chemoprevention Program (to A.D.K.).