Title

Surface-Bound Cytomimetic Assembly Based on Chemoselective and Biocompatible Immobilization and Further Modification of Intact Liposome

Document Type

Article

Publication Date

11-17-2010

Publication Title

Bioconjugate Chemistry

Abstract

A surface-bound cytomimetic assembly based on chemically selective and biocompatible immobilization and further modification of intact liposome is described. Liposomes carrying PEG-triphenylphosphine were chemoselectively immobilized onto azide-modified glass slides through Staudinger ligation, followed by modification with azide-modified lactose as a model biomolecule through Staudinger ligation to afford the surface-bound cytomimetic assembly. The intact liposome immobilized and modified and its protein binding activity were confirmed by fluorescence imaging, fluorescent dye releasing kinetics, and AFM techniques. The resultant surface-bound cytomimetic assembly showed sustained stability and fluorescent dye releasing kinetics and specific protein binding activity. The reported method provides a robust platform for preparation of a complex immobilized liposome system with multifunctional components, which mimics the cell surface in both geographical and content features and thus will find important biomedical applications.

Comments

This work was financially supported by the grant from NIH
(1R01HL102604-01), American Health Assistance Foundation
(AHAF-H2007027), and the Startup Fund from Cleveland
State University.

DOI

10.1021/bc100220j

Volume

21

Issue

11