Surface-Bound Cytomimetic Assembly Based on Chemoselective and Biocompatible Immobilization and Further Modification of Intact Liposome
Document Type
Article
Publication Date
11-17-2010
Publication Title
Bioconjugate Chemistry
Abstract
A surface-bound cytomimetic assembly based on chemically selective and biocompatible immobilization and further modification of intact liposome is described. Liposomes carrying PEG-triphenylphosphine were chemoselectively immobilized onto azide-modified glass slides through Staudinger ligation, followed by modification with azide-modified lactose as a model biomolecule through Staudinger ligation to afford the surface-bound cytomimetic assembly. The intact liposome immobilized and modified and its protein binding activity were confirmed by fluorescence imaging, fluorescent dye releasing kinetics, and AFM techniques. The resultant surface-bound cytomimetic assembly showed sustained stability and fluorescent dye releasing kinetics and specific protein binding activity. The reported method provides a robust platform for preparation of a complex immobilized liposome system with multifunctional components, which mimics the cell surface in both geographical and content features and thus will find important biomedical applications.
Recommended Citation
Ma, Y.; Zhang, H.; Sun, X. Surface-Bound Cytomimetic Assembly Based on Chemoselective and Biocompatible Immobilization and Further Modification of Intact Liposome. Bioconjugate Chem. 2010, 21, 1994-1999.
DOI
10.1021/bc100220j
Volume
21
Issue
11
Comments
This work was financially supported by the grant from NIH
(1R01HL102604-01), American Health Assistance Foundation
(AHAF-H2007027), and the Startup Fund from Cleveland
State University.