Title

Synthesis and Characterization of Biotin Chain-End Functionalized Boronic Acid-Containing Polymer (Boropolymer) as Functional Glyco-Affinity Macroligand

Document Type

Article

Publication Date

7-2010

Publication Title

Reactive and Functional Polymers

Abstract

A biotin chain-end functionalized boronic acid-containing polymer (biotin boropolymer) as an oriented glyco-affinity macroligand for efficient carbohydrate and glycoconjugate purification and identification application was described. Briefly, the biotin boropolymer was synthesized via a biotin derivated arylamine initiated cyanoxyl-mediated free-radical polymerization in one-pot fashion. The specific streptavidin binding capacity of biotin boropolymer was confirmed by streptavidin–HABA assay, while the specific carbohydrate binding capacity of biotin boropolymer was evaluated by Alizarin Red S binding assay. Efficient glyco-capturing followed by direct MALDI mass spectrometry identification of the captured carbohydrate was demonstrated by using magnetic bead functionalized with the biotin boropolymer via streptavidin/biotin interaction.

Comments

This work was supported by Grants from American Health Assistance Foundation (AHAF-H2007027) and Cleveland State University Faculty Research Development Grant.

DOI

10.1016/j.reactfunctpolym.2010.03.005

Volume

70

Issue

7