On the Orders of Magnitude of Epigenic Dynamics and Monoclonal Antibody Production

Authors

Joanne M. Savinell (Belovich), Cleveland State UniversityFollow
Gyun Min Lee, University of Michigan
Bernhard O. Palsson, University of Michigan

Document Type

Article

Publication Date

9-1989

Publication Title

Bioprocess Engineering

Abstract

The hybridoma cell's maximum capacity for monoclonal antibody (MAb) production is estimated to be 2300–8000 MAb molecules/cell/s, using measured rates of transcription and translation, and the limitations imposed by the size of the polymerase molecule and the ribosome. Nearly all the production rates reported in the literature fall into or below this range of production rates. Data from batch cultures of hybridomas demonstrate a constant specific rate of MAb production until the time integral of the viable cell concentration reaches about 10⁸ cells · h/cm³. At this point, some essential nutrients from the standard media are depleted, causing MAb production to decline.

Comments

The authors acknowledge support for this research from the National Science Foundation grant EET-8712756 and from the Presidential Initiatives Fund at the University of Michigan grant 135-PIS.

Repository Citation

Savinell (Belovich), Joanne M.; Lee, Gyun Min; and Palsson, Bernhard O., "On the Orders of Magnitude of Epigenic Dynamics and Monoclonal Antibody Production" (1989). Chemical & Biomedical Engineering Faculty Publications. 158.
https://engagedscholarship.csuohio.edu/encbe_facpub/158

Volume

4

Issue

5

DOI

10.1007/BF00369177

Version

Postprint

Publisher's Statement

https://link.springer.com/article/10.1007/BF00369177