Biosensors & Bioelectronics
The field-effect enzymatic detection technique has been applied to the amperometric immunoassay of the cancer biomarker, carcinoma antigen 125 (CA 125). The detection adopted a reagentless approach, in which the analyte, CA 125, was immobilized on the detecting electrode, which was modified using carbon nanotubes, and the detection signal was obtained by measuring the reduction peak current of the enzyme that was used to label the antibody. A gating voltage was applied to the detecting electrode, inducing increase in the signal current and therefore providing amplification of the detection signal. The voltage-controlled signal amplification of the detection system has increased the sensitivity and lowered the detection limit of the system. A detection limit of 0.9U/ml was obtained in the work.
Wang, JIapeng and Yau, Siu-Tung, "Field-Effect Amperometric Immuno-Detection of Protein Biomarker" (2011). Electrical Engineering & Computer Science Faculty Publications. 63.
Wang, J., , & Yau, S. (2011). Field-effect amperometric immuno-detection of protein biomarker. Biosensors and Bioelectronics, 29(1), 210-214. doi:10.1016/j.bios.2011.07.072
NOTICE: this is the author’s version of a work that was accepted for publication in Biosensors & Bioelectronics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biosensors & Bioelectronics, 29, 1, (11-15-2011); 10.1016/j.bios.2011.07.072