Date of Award

Spring 5-3-2022

Degree Type

Thesis

Degree Name

Master of Science in Biology

Department

Biological, Geological and Environmental Sciences

First Advisor

Kondratov, Roman

Second Advisor

Weyman, Crystal

Third Advisor

Gulshan, Kailash

Subject Headings

Physiology

Abstract

The Caloric Restriction (CR) diet in mammals has been shown to increase longevity and prevent metabolic disorders. Some of the benefits of CR include improved glucose homeostasis, namely improvements in glucose tolerance. Mice on the CR diet have a 30% reduction in food provided, as well as a fasting period between meals as they are only fed once a day. Hence the benefits of CR stem from both fasting and reduced calorie intake. In this study, we investigated if the fasting component of CR is responsible for the benefits of this diet in improving glucose homeostasis. In particular, we used glucose tolerance tests to show that varying durations of fasting affect the glucose tolerance of fasted mice differently. Shorter fasts improved glucose tolerance, while longer fasts showed similar or reduced tolerance compared to non-fasted mice. Ultimately, we observed that fasting may be responsible for a part of the benefits of CR, but it is not the only contributor to the advanced glucose tolerance and homeostasis of mice on CR.

Included in

Physiology Commons

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