Date of Award
Biological, Geological and Environmental Sciences
Bone cells, Myoblasts, Apoptosis, Cell differentiation, Cell cycle -- Regulation, Messenger RNA, Cellular signal transduction, PUMA, Apoptosis, Translation, mRNA, IRES, Skeletal Myoblast, Differentiation, Total protein synthesis
Differentiation and apoptosis are coordinately regulated in skeletal myoblasts. During the process of skeletal myoblast differentiation, our lab has determined that roughly 30 of myoblasts undergo apoptosis rather than differentiation. Further, we have reported that cytochrome C is released, and that the expression level of the pro-apoptotic Bcl2 family member PUMA is elevated, when 23A2 myoblasts are cultured in DM. Our long-term goal is to identify targets for manipulation that would abrogate this apoptosis without affecting differentiation. These findings could be significant to the study of myoblast transfer as a therapeutic approach. The goals of this dissertation are to: 1- determine the significance of PUMA in apoptosis associated with skeletal myoblast differentaion 2- determine the mechanism responsible for the increased expression of PUMA
Shaltouki, Atossa, "Novel Translational Regulation of the Proapoptotic bcl2 Member Puma and Its Role During Skeletal Myoblast Apoptosis" (2011). ETD Archive. 266.