Document Type
Article
Publication Date
11-6-2019
Publication Title
Small
Abstract
Dynamic DNA nanodevices are designed to perform structure‐encoded motion actuated by a variety of different physicochemical stimuli. In this context, hybrid devices utilizing other components than DNA have the potential to considerably expand the library of functionalities. Here, the reversible reconfiguration of a DNA origami structure using the stimulus sensitivity of elastin‐like polypeptides is reported. To this end, a rectangular sheet made using the DNA origami technique is functionalized with these peptides and by applying changes in salt concentration the hydrophilic–hydrophobic phase transition of these peptides actuate the folding of the structure. The on‐demand and reversible switching of the rectangle is driven by externally imposed temperature oscillations and appears at specific transition temperatures. Using transmission electron microscopy, it is shown that the structure exhibits distinct conformational states with different occupation probabilities, which are dependent on structure‐intrinsic parameters such as the local number and the arrangement of the peptides on the rectangle. It is also shown through ensemble fluorescence resonance energy transfer spectroscopy that the transition temperature and thus the thermodynamics of the rectangle‐peptide system depends on the stimuli salt concentration and temperature, as well as on the intrinsic parameters.
Repository Citation
Goetzfried, Marisa A.; Vogele, Kilian; Mückl, Andrea; Kaiser, Marcus; Holland, Nolan B.; Simmel, Friedrich C.; and Pirzer, Tobias, "Periodic Operation of a Dynamic DNA Origami Structure Utilizing the Hydrophilic–Hydrophobic Phase‐Transition of Stimulus‐Sensitive Polypeptides" (2019). Chemical & Biomedical Engineering Faculty Publications. 166.
https://engagedscholarship.csuohio.edu/encbe_facpub/166
Volume
15
Issue
45
DOI
10.1002/smll.201903541
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Comments
Open Access Article
The authors gratefully acknowledge financial support by the TUM International Graduate School for Science and Engineering IGSSE project no. 9.05 (to M.A.G. and T.P.), the DFG through SFB 1032 Nanoagents (TP A2) and the Cluster of Excellence Nanosystems Munich (NIM).