Document Type
Article
Publication Date
9-2010
Publication Title
Biotechnology Progress
Abstract
Accurate cell settling velocity determination is critical for perfusion culture using a gravity settler for cell retention. We have developed a simple apparatus (a 'settling column') for measuring settling velocity and have validated the procedure with 15-μm polystyrene particles with known physical properties. The measured settling velocity of the polystyrene particles is within 4% of the value obtained using the traditional Stokes' law approach. The settling velocities of three hybridoma cell lines were measured, resulting in up to twofold variation among cell lines, and the values decreased as the cell culture aged. The settling velocities of the nonviable cells were 33-50% less than the corresponding viable cells. The significant variation of settling velocities among cell populations and growth phases confirms the necessity of routine measurement of this property during long-term perfusion culture.
Repository Citation
Wang, Zhaowei and Belovich, Joanne M., "A Simple Apparatus for Measuring Cell Settling Velocity" (2010). Chemical & Biomedical Engineering Faculty Publications. 46.
https://engagedscholarship.csuohio.edu/encbe_facpub/46
Original Citation
Zhaowei, W., & Belovich, J. M. (2010). A simple apparatus for measuring cell settling velocity. Biotechnology Progress, 26(5), 1361-1366. doi:10.1002/btpr.432
Volume
26
Issue
5
DOI
10.1002/btpr.432
Version
Postprint
Publisher's Statement
This is the accepted version of the following article: Zhaowei, W., & Belovich, J. M. (2010). A simple apparatus for measuring cell settling velocity. Biotechnology Progress, 26(5), 1361-1366. doi:10.1002/btpr.432, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/btpr.432/abstract
