Detection of a-Methylacyl-CoA Racemase in Serum and Urine Using a Highly Sensitive Electrochemical Immunodetector
Document Type
Article
Publication Date
6-12-2014
Publication Title
Electroanalysis
Abstract
The detection of α-methylacyl-CoA racemase (AMACR), a novel biomarker for prostate cancer, is demonstrated in serum and urine using a novel immuno-detection method. The detection system consists of a three-electrode conventional electrochemical cell modified with a gating electrode for applying a gating voltage VG to the immune complex immobilized on the working electrode to provide signal amplification. The detection system is realized by integrating gating electrodes with screen-printed electrodes. This detection method does not require involved sample preparation procedures. The detection was demonstrated in serum and urine samples on the nanogram/mL level with VG equal to 0.6 V. Detection in serum was also performed on the picogram/mL level with a limit of 100 picogram/mL with VG=0.6 V being a necessary condition.
Repository Citation
Wang, Jiapeng and Yau, Siu-Tung, "Detection of a-Methylacyl-CoA Racemase in Serum and Urine Using a Highly Sensitive Electrochemical Immunodetector" (2014). Electrical and Computer Engineering Faculty Publications. 309.
https://engagedscholarship.csuohio.edu/enece_facpub/309
DOI
10.1002/elan.201400123
Volume
26
Issue
7
