Date of Award

2018

Degree Type

Dissertation

Degree Name

Doctor of Philosophy in Clinical-Bioanalytical Chemistry

Department

Chemistry

First Advisor

Yan, Xu

Subject Headings

Analytical Chemistry, Chemistry

Abstract

The ever-growing need to develop new anticancer agents due to increased incidence of cancer and its recurrence has led to a broad range of investigational efforts in anticancer research. Drug discovery and development processes involve evaluations of pharmacokinetic and pharmacodynamic parameters of potential drug candidates in order to assess their safety, toxicity, and efficacy profiles. By providing sensitivity, assay precision, reliability, and high-throughput, mass spectrometry has become an indispensable tool in quantitative and qualitative analysis, from early-stage drug discovery and development processes to routine laboratory analysis. Development of bioanalytical methods requires knowledge of separation science with instrumentation, data interpretation and sample preparation techniques from complex biological matrices. This work describes the theory, instrumentation, and method development processes behind these investigations. Further, detailed method development and validation procedures for investigational anticancer agents MLN0128, GS87, and curcumin using liquid chromatography-tandem mass spectrometry were presented.

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