Effects of Magnesium Sulfate, Digestate and Other Inorganic Nutrients on the Phototrophic Growth of the Green Microalga Scenedesmus Dimorphus
Date of Award
Chemical and Biomedical Engineering
Microalgae -- Biotechnology, Microalgae -- Cultures and culture media, Scenedesmus, Photosynthesis, Biomass energy -- Research
The individual components of the growth media, notably magnesium, have a profound impact on the growth and lipid production of the green microalgae, Scenedesmus dimorphus. The goal of this work was to investigate these effects and explain the causes via multiple experiments. It is important to understand the aspects of algal growth because they have a significant impact on the commercial viability of microalgal lipids as a potential feedstock for biofuel production. Digestate, derived from bovine waste and a potential cost effective nutrient replacement for conventional media types, was found to maximize growth and lipid concentration in S. dimorphus at 1.25 and 1.75 in water respectively and that increasing Mg concentration increased both growth and maximum cell density. The second experiment derived the Monod parameters ks (33 ± 7 mg/L) and umax (0.59 ± 0.04 days-1) for magnesium limited growth. The third experiment utilized ICP spectral analysis to monitor nutrient consumption over time allowing for the calculation of biomass yields for Mg (476 ± 132), Ca (247 ± 57.8), Fe (8550 ± 824), Mn (5310 ± 1450) all in g/g
Schwenk, Jacob R., "Effects of Magnesium Sulfate, Digestate and Other Inorganic Nutrients on the Phototrophic Growth of the Green Microalga Scenedesmus Dimorphus" (2012). ETD Archive. 353.