Serum Can Act as a Shear Protecting Agent in Agitated Hybridoma Cell Cultures

Authors

Gyun Min Lee, University of Michigan
Joanne M. Savinell (Belovich), Cleveland State UniversityFollow
Bernhard O. Palsson, University of Michigan

Document Type

Article

Publication Date

12-1989

Publication Title

Hybridoma

Abstract

Hybridoma cells, S3H5/γ2bA2, were grown in spinner flasks containing RPMI 1640 media at different levels of serum. Cells in low serum media (1% fetal bovine serum) were more sensitive to shear induced by mechanical agitation than cells in high serum media (10%), indicating that serum can protect cells from shear induced by mechanical agitation. The data suggest that serum alters the physiological properties of the cells to make them more shear-resistant, rather than by changing the physico-chemical properties of medium.

Repository Citation

Lee, Gyun Min; Savinell (Belovich), Joanne M.; and Palsson, Bernhard O., "Serum Can Act as a Shear Protecting Agent in Agitated Hybridoma Cell Cultures" (1989). Chemical & Biomedical Engineering Faculty Publications. 157.
https://engagedscholarship.csuohio.edu/encbe_facpub/157

Volume

8

Issue

6

DOI

10.1089/hyb.1989.8.639