Document Type
Article
Publication Date
9-8-2025
Publication Title
ACS Biomaterials Science and Engineering
Abstract
Peripheral nerve injuries (PNIs) have a significant impact on the quality of life for patients suffering from trauma or disease. In injuries with critical nerve gaps, PN regeneration requires tissue scaffolds with appropriate physiological properties that promote cell growth and functions. Hydrogel scaffolds represent a promising platform for engineering soft tissue constructs that meet key physiological requirements. Nonetheless, ongoing innovation remains essential, as current designs continue to fall short of replicating the functional performance of autografts in bridging critical-sized nerve defects. In this study, gelatin methacrylate (gelMA)-based hydrogels are evaluated to fully characterize their pore structure, compressive stiffness, viscoelasticity, and 3D bioprintability. Hyaluronic acid (HA) and single-walled carbon nanotubes (SWCNTs) are explored as gelMA additives to modify viscoelastic and electrically conductive properties, respectively. Finally, Schwann cell (SC) and human umbilical vein endothelial cell (HUVEC) growth and functions are quantified to assess the biocompatibility of the hydrogel composites as materials for nerve scaffold fabrication. It was found that the microstructure and mechanical properties of gelMA-based hydrogels can be precisely controlled by modifying the concentrations of each component. The addition of HA led to altered viscoelastic properties of the cured structures and SWCNTs increased electrical conductivity, with both additives maintaining cytocompatibility while influencing the protein expression of both SCs and HUVECs. These composite hydrogels have potential in PNI regeneration applications.
Repository Citation
Schmitz, Kylie M.; Larson, Tanner L.; Borovich, Michael W.; Wu, Xianfang; Ao, Geyou; Jack, Megan; and Ning, Liqun, "Development of GelMA-Based Hydrogel Scaffolds with Tunable Mechanical Properties for Applications in Peripheral Nerve Regeneration" (2025). Chemical & Biomedical Engineering Faculty Publications. 245.
https://engagedscholarship.csuohio.edu/encbe_facpub/245
Volume
11
Issue
9
DOI
10.1021/acsbiomaterials.5c00023
Version
Publisher's PDF
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Comments
This research was primarily funded by the Ning lab via the Faculty Startup Program and Faculty Research and Development Grant through Cleveland State University. Additional support was provided by the National Science Foundation under Grants Nos. 2433244 and 2501849 (Ning Lab), 1126126 (SEM), CMMI-2118416 (Ao Lab), and CAREER-2142579 (Ao Lab), as well as NIH 1 S10 OD010381 (Confocal Microscope).