Date of Award

2008

Degree Type

Thesis

Department

Chemical and Biomedical Engineering

First Advisor

Belovich, Joanne

Subject Headings

Orthopedic implants, Titanium, Bone cells, Titanium -- Metallurgy, Titanium castings, Osteoblast cells, Polished and anodized titanium, Polished and NaOH treated titanium, Polished titanium, Titanium, UMR 106-0

Abstract

Adhesion and proliferation of UMR 106-01 osteoblast cells were studied on various surface modified titanium materials such as polished, sandblasted, anodized and alkaline treated. Anodization of polished surface in Hydrofluoric acid developed nano-tubes, while NaOH treatment produced spongy microporous morphology. Test samples were coated with non-adhesive protein bovine serum albumin and compared with fibronectin coated specimens. The adhesion study lasted for 4 hrs, where osteoblast cells were cultured in serum free medium. Polished titanium, anodized titanium and NaOH titanium have shown similar percentages of cell adherence. The proliferation study lasted for 48 hrs, where cells were initially allowed to adhere to the surface in serum free medium for 4 hrs, followed by a medium change to 10 fatal bovine serum. The specific growth rate after 48 hrs in culture on the polished surface was found to be comparable to the tissue culture plastic, which exhibited a high growth rate. No significant difference was found in cell numbers between polished, anodized and NaOH-Ti, but each has varying cell orientation on the surface. Fluorescence images stained with alkaline phosphatase revealed that polished surface had cells flattened to the surface with short filapodia. Anodized surface had cells uniformly distributed across the surface where as NaOH-Ti displayed cells in colonies. Cells were found bonding to the surface of NaOH-Ti firmly using their filapodia as an anchoring agent. These results suggest that NaOH-Ti provides support in initial hours of implantation and bolsters cell proliferation. All together this process may help to better integrate titanium implant surfaces

Download
COinS