Thermal Behavior and Signature Patterns of Human Cytokine and Soluble Cytokine Receptors Investigated Using Dielectric Thermal Analysis and Thermogravimetry
Document Type
Article
Publication Date
4-1-2012
Publication Title
Journal of Thermal Analysis and Calorimetry
Abstract
Cytokines are small regulatory proteins secreted mostly by cells of the immune system. Cytokines participate in anti-inflammatory and pro-inflammatory processes in the body and in responses to host exposure to pathogens. In this study, the thermal behavior of human recombinant cytokines and soluble cytokine receptors; IFNγ, TNFα, IL-1 receptor antagonist, soluble TNF-receptor types 1 and 2, and sIL-2 receptor α were analyzed by dielectric thermal analysis at 37 °C and by thermogravimetry. Measurements were performed at a frequency range of 0.1-300,000 Hz. Permittivity and loss factor measurements were used to calculate mobility of charges (tan δ values) in the proteins from Debye plots. Peak frequencies and polarization times were used to determine dielectric signatures for each cytokine and receptor. Peak frequencies and polarization times were obtained for each cytokine and receptor analyzed. Detection of unique dielectric signatures of the proteins will aid development of sensitive dielectric sensors capable of detecting cytokines and soluble cytokine receptors in various human samples for malaria diagnosis. © 2011 Akadémiai Kiadó, Budapest, Hungary.
DOI
10.1007/s10973-011-1530-9
Recommended Citation
Saleh, Salaam W.; Moreno-Molek, Susan E.; Mantheni, Druthiman Reddy; Maheswaram, Manik Pavan Kumar; Sam-Yellowe, Tobili; and Riga, Alan T., "Thermal Behavior and Signature Patterns of Human Cytokine and Soluble Cytokine Receptors Investigated Using Dielectric Thermal Analysis and Thermogravimetry" (2012). Biological, Geological, and Environmental Faculty Publications. 213.
https://engagedscholarship.csuohio.edu/scibges_facpub/213
Volume
108
Issue
1