Journal of Chromatography B: Biomedical Sciences and Applications
Lysophosphatidic acid (LPA) is the simplest form of lysophospholipid. Molecular species of LPA have been identified as the potent components in the ovarian cancer activation factor. The elevated plasma LPAs may be used as potential biomarkers for the early detection of ovarian cancer. This paper is the first report on the quantitative analysis of molecular species of LPA using capillary electrophoresis. In this work, the separation of LPAs was achieved within 14 min in an adenosine monophosphate-borate–methanol–water solution, and the measurement was accomplished by indirect UV detection. With LPA (D) as internal standard, the method had linear calibration ranges for LPAs from 2.8 to 75 μM. The detection limits for various molecular species of LPA were from 1.2 to 2.3 μM by the pressure injection at 3.45 kPa for 5 s. The method had been applied to serum fortified with LPA (S), LPA (O), LPA (P), and LPA (M) and the recoveries ranged from 83 to 112%.
Chen, Yi Lung and Xu, Yan, "Determination of Lysophosphatidic Acids by Capillary Electrophoresis with Indirect Ultraviolet Detection" (2001). Chemistry Faculty Publications. 178.
NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Chromatography B: Biomedical Sciences and Applications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Chromatography B: Biomedical Sciences and Applications, 753, 2, April 5, 2001 DOI#10.1016/S0378-4347(00)00582-X