Analytica Chimica Acta
Capillary enzyme immunoassay with flow-injection analysis for digoxin using the sequential saturation technique has been developed. Glass capillary tubes (10 cm × 0.53 mm i.d.) with immobilized digoxin antibody were used as the immunoassay reactor. The product of enzymatic reaction. 4-aminophenol, was detected amperometrically. The digoxin and the labeled digoxin binding reaction with the immobilized digoxin antibody were completed in 2 and 10 min, respectively. Digoxin was determined in a 20-μl sample with a detection limit of 10 pg ml−1 (200 fg or 260 attomoles) and a 3 orders of magnitude range.
Kaneki, Noriaki; Xu, Yan; Kumari, Anu; Halsall, H. Brian; Heineman, William R.; and Kissinger, Peter T., "Electrochemical Enzyme Immunoassay Using Sequential Saturation Technique in A 20-μl Capillary: Digoxin as A Model Analyte" (1994). Chemistry Faculty Publications. 187.
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