Journal of Chromatography B: Biomedical Sciences and Applications
Trihexyphenidyl, biperiden and procyclidine are anticholinergic drugs produced as racemates for the treatment of Parkinson’s disease. This paper describes a simple and sensitive LC–MS method for the simultaneous determination of these compounds in human serum. An on-line sample clean-up procedure was used, where serum samples were directly injected into a “restricted-access media” pre-column. After the exclusion of the serum proteins, the drug molecules were eluted to a β-cyclodextrin analytical column for chiral separation. The quantitation was done by electrospray ionization MS using diphenidol as an internal standard. The method developed has limits of detection of 1 ng/ml, at least two-orders-of-magnitude linear dynamic ranges (r>0.999), and RSDs of less than 10%. The system can be completely automated for increased sample throughput and unattended analyses.
Čápka, Vladimı́r and Xu, Yan, "Simultaneous Determination of Enantiomers of Structurally Related Anticholinergic Analogs in Human Serum by Liquid Chromatography–Electrospray Ionization Mass Spectrometry with On-Line Sample Cleanup" (2001). Chemistry Faculty Publications. 212.
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NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Chromatography B: Biomedical Sciences and Applications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Chromatography B: Biomedical Sciences and Applications, 762, 2, October 25, 2001. DOI#10.1016/S0378-4347(01)00364-4