Document Type
Article
Publication Date
11-2-1999
Publication Title
Journal of Pharmaceutical and Biomedical Analysis
Abstract
The antiparkinsonian drug trihexyphenidyl (THP) is currently manufactured and administered as a racemate. However, stereochemistry can play significant role in the drug’s pharmacokinetics, biotransformation, metabolism, interaction with cellular and tissue components and overall effect on human body. It is necessary to consider such a drug as a mixture of two compounds (drug enantiomers), with their own effect on the human body. The present paper describes a simple and sensitive LC–MS method for the stereoselective determination of THP in human serum. In this study, the sample was prepared by a solid-phase extraction (SPE) procedure. The enantiomer separation was done using native β-cyclodextrin stationary phase LC column. The combination of ESI–MS detection and SPE showed excellent sensitivity and selectivity of the method. The limits of detection of <0.1 ng/ml can be easily achieved, which is 7000 times lower than the detection limits achievable by a UV detection method. The method has at least a 3-order of magnitude linear dynamic range for both enantiomers (concentrations up to 1323 ng/ml were tested). This is 24 times wider than the therapeutic range of THP (peak THP plasma concentration of 55 ng/ml was previously reported). The recoveries of THP enantiomers from the human serum were >95%.
Recommended Citation
Čápka, Vladimı́r; Xu, Yan; and Chen, Yong Hong, "Stereoselective Determination ofTrihexyphenidyl in Human Serum by LC–ESI–MS" (1999). Chemistry Faculty Publications. 216.
https://engagedscholarship.csuohio.edu/scichem_facpub/216
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
DOI
10.1016/S0731-7085(99)00116-8
Version
Postprint
Volume
21
Issue
3
