Document Type
Article
Publication Date
8-1-2004
Publication Title
Analytical and Bioanalytical Chemistry
Abstract
Standard calibration methods used to determine trace analytes usually yield significant deviations from the actual analyte value in the presence of interferents in the assay media. These deviations become of particular concern when the concentration of the analyte is low, and when the results are used to draw mechanistic or kinetic conclusions, for instance in enzyme structure-function studies. In these circumstances, the H-point standard addition method (HPSAM) provides superior precision and accuracy. This method is developed here for the case of the spectrophotometric Griess assay used to determine nitrite in various enzymology investigations, such as nitrite determination in studies of nitrite reductases (NiR), or when determining nitrite as a breakdown product of nitric oxide synthesized by NOS enzymes. The results obtained by HPSAM are contrasted with those of the traditional calibration method.
Recommended Citation
Indika, Perera N. and Bayachou, Mekki, "Eliminating Absorbing Interference Using The H-point Standard Addition Method: Case of Griess Assay in The Presence of Interferent Heme Enzymes Such As NOS" (2004). Chemistry Faculty Publications. 312.
https://engagedscholarship.csuohio.edu/scichem_facpub/312
DOI
10.1007/s00216-004-2674-2
Version
Postprint
Publisher's Statement
The final publication is available at Springer via http://dx.doi.org/10.1007/s11661-004-0269-3
Volume
379
Issue
7
