Pharmacokinetic Study of an Anti-trypanosome Agent with Different Formulations and Administration Routes in Mice by HPLC-MS/MS
Bin Su: https://orcid.org/0000-0002-0711-441X
Previously compound 12 showed great anti-trypanosome activity without toxicity in an in vivo study. In the current study, a sensitive and rapid high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed and validated to investigate its pharmacokinetics in mouse plasma. A protein precipitation method was applied to extract the compound, and it was then separated using a Kinetex C-18 column with mobile phase consisting of acetonitrile-0.1% formic acid water (50:50, v/v) at a flow rate of 300 mu l/min. The analytes were detected with the multiple reaction monitoring in negative electrospray ionization source for quantitative response of the compounds. Compound 12 was detected at m/z 477.0 -> 367.2, while the internal standard compound 14 was detected at m/z 499.2 -> 268.2. Inter- and intra-day precision was
Li, Yaxin; Orahoske, Cody M.; Dano, Raina; Zhang, Wenjing; Li, Bibo Ph.D.; and Su, Bin Ph.D., "Pharmacokinetic Study of an Anti-trypanosome Agent with Different Formulations and Administration Routes in Mice by HPLC-MS/MS" (2021). Chemistry Faculty Publications. 559.