Pharmacokinetic Study of an Anti-trypanosome Agent with Different Formulations and Administration Routes in Mice by HPLC-MS/MS

ORCID ID

Bin Su: https://orcid.org/0000-0002-0711-441X

Document Type

Article

Publication Date

5-2021

Publication Title

Biomedical Chromatography

Abstract

Previously compound 12 showed great anti-trypanosome activity without toxicity in an in vivo study. In the current study, a sensitive and rapid high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed and validated to investigate its pharmacokinetics in mouse plasma. A protein precipitation method was applied to extract the compound, and it was then separated using a Kinetex C-18 column with mobile phase consisting of acetonitrile-0.1% formic acid water (50:50, v/v) at a flow rate of 300 mu l/min. The analytes were detected with the multiple reaction monitoring in negative electrospray ionization source for quantitative response of the compounds. Compound 12 was detected at m/z 477.0 -> 367.2, while the internal standard compound 14 was detected at m/z 499.2 -> 268.2. Inter- and intra-day precision was

Comments

National Institute of Allergy and Infectious Diseases, Grant/Award Number: R15AI 103889; National Science Foundation, Grant/Award Numbers: CHE-0923398, CHE1126384

DOI

10.1002/bmc.5169

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