Function of a Novel Checkpoint Protein in the Germ Line

Authors

Steven Drellishak, Cleveland State University
Marina Bykova, Cleveland State University
G. Valentin Börner, Cleveland State University

Files

Description

Successful reproduction of Saccharomyces cerevisiae relies on the organism’s ability to complete the meiotic cell cycle and produce viable gametes. Zip1 is a protein that constitutes the central component of a protein structure that connects homologous chromosomes known as the synaptonemal complex. Zip1 is important for progression through the meiotic cell cycle. The C terminus of the coiled-coil Zip 1 protein is responsible for localization to the axes of the chromosomes. An internal deletion near the C terminus of Zip1, called zip1-c1, yields a stronger meiotic arrest than a mutation where Zip1 is completely deleted. The more efficient meiotic progression in a Zip1 deletion mutation versus the zip1-c1 mutant suggests that zip1-c1 prevents an alternative pathway of meiotic progression. A genomic screen of the Nasmyth genomic library revealed candidate plasmids N5 and N89 containing yeast genes which, when overexpressed, increase spore viability and bypass meiotic arrest in the zip1-c1 mutant. This has implications that the genes on the overexpression plasmids serve some function in correcting mistakes in meiosis when Zip1 is mutated.

Publication Date

9-5-2013

Disciplines

Cell and Developmental Biology | Genetics | Life Sciences

Comments

Student Researchers: Steven Drellishak; Marina Bykova

Faculty Advisor: G. Valentin Börner, Ph.D.

Recommended Citation

Drellishak, Steven; Bykova, Marina; and Börner, G. Valentin, "Function of a Novel Checkpoint Protein in the Germ Line" (2013). Undergraduate Research Posters 2013. 15.
https://engagedscholarship.csuohio.edu/u_poster_2013/15