Selective Plane Illumination Microscopy

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Author

David Ian Pendleton, Cleveland State University

Faculty Advisors

Maitland, Kristen

Description

Selective plane illumination microscopy (SPIM), or light sheet microscopy, is a microscopy technique that allows you to acquire high resolution fluorescence images of biological samples by illuminating the sample with a thin plane from the side, instead of along the imaging axis as in traditional transillumination or epi-illumination. The purpose of this SPIM research assignment was to combine two previously built systems, an inverted SPIM and a tunable lens system. This report includes use of optics, coupling lasers and proper technique to building optical systems. Programming in Matlab, LabVIEW, and other programming languages was used to synchronize the shutter and camera electronics and acquire and process images. The paper is concluded with expected results to ensure to detection path is optimized.

Publication Date

2015

College

Washkewicz College of Engineering

Disciplines

Engineering

Recommended Citation

Pendleton, David Ian, "Selective Plane Illumination Microscopy" (2015). Undergraduate Research Posters 2015. 64.
https://engagedscholarship.csuohio.edu/u_poster_2015/64

Student Publication

This item is part of the McNair Scholars Program.