Liquid Chromatography–Mass Spectrometry Method for The Analysis of The Anti-Cancer Agent Capecitabine and Its Nucleoside Metabolites in Human Plasma

Authors

Yan Xu, Cleveland State UniversityFollow
Jean L. Grem, National Cancer Institute at the National Naval Medical Center

Document Type

Article

Publication Date

1-5-2003

Publication Title

Journal of Chromatography B

Abstract

A reversed-phase high-performance liquid chromatography method with electrospray ionization and mass spectral detection is described for the determination of capecitabine, 5′-deoxy-5-fluorocytidine and 5′-deoxy-5-fluorouridine in human plasma with 5-chloro-2′-deoxyuridine as the internal standard. An on-line sample clean-up procedure allows dilution of the plasma sample with the initial mobile phase. The linear dynamic range is 0.0500–10.0 μg/ml for capecitabine, and 0.0500–25.0 μg/ml for the metabolites, 5′-deoxy-5-fluorocytidine and 5′-deoxy-5-fluorouridine, respectively. This method has been used to analyze plasma samples from patients receiving capecitabine in combination with oxaliplatin.

Recommended Citation

Xu, Yan and Grem, Jean L., "Liquid Chromatography–Mass Spectrometry Method for The Analysis of The Anti-Cancer Agent Capecitabine and Its Nucleoside Metabolites in Human Plasma" (2003). Chemistry Faculty Publications. 199.
https://engagedscholarship.csuohio.edu/scichem_facpub/199

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

DOI

10.1016/S1570-0232(02)00674-8

Version

Postprint

Volume

783

Issue

1