Document Type
Article
Publication Date
6-12-2025
Publication Title
Frontiers in Bioengineering and Biotechnology
Abstract
Therapeutic tissue regeneration remains a significant unmet need in heart failure and cardiovascular disease treatment, which are among the leading causes of death globally. Decellularized heart matrix (DHM) offer promising advantages for tissue engineering, including low immunogenicity and seamless integration into biological processes, facilitating biocompatibility. However, DHM is challenged by weak mechanical properties that limit its utility to biomedical applications like tissue engineering. To address this limitation, we functionalized DHM with methacryloyl functional groups (DHMMA) that support UV-induced crosslinking to enhance mechanical properties. By modulating the degree of methacryloyl substitution, a broad range of stiffness was achieved while maintaining cell viability on crosslinked DHMMA. Additionally, we show that increasing UV exposure time and pH increases DHMMA stiffness. Furthermore, topographical features transferred on DHMMA via soft lithography facilitated physical orientation of cells in culture. We demonstrate DHMMA as a scaffold with tunable stiffness and matrix-degradation properties suitable for cell survival and microfabrication for cardiac tissue engineering applications.
Repository Citation
Pierre, Valinteshley; Wu, Douglas H.; Liu, Chao; Ertugral, Elif; Kothapalli, Chandrasekhar R.; and Senyo, Samuel E., "Tunable Methacrylated Decellularized Heart Matrix: A Versatile Scaffold for Cardiac Tissue Engineering" (2025). Chemical & Biomedical Engineering Faculty Publications. 248.
https://engagedscholarship.csuohio.edu/encbe_facpub/248
Original Citation
Pierre V, Wu DH, Liu C, Ertugral E, Kothapalli C and Senyo SE (2025) Tunable methacrylated decellularized heart matrix: a versatile scaffold for cardiac tissue engineering. Front. Bioeng. Biotechnol. 13:1579246. doi: 10.3389/fbioe.2025.1579246
Volume
13
DOI
10.3389/fbioe.2025.1579246
Version
Publisher's PDF
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Comments
This work was supported by R25 HL145817, Taipei Medical University/CWRU Translational Collaborative Award to SS, partial support from National Science Foundation (NSF) grants 1927602 and 1337859 to CK, CWRU Biomedical Imaging T32 5T32EB007509, and Medical Scientist Training Program 5T32GM007250, 5TL1TR002549 to DW.