Trafficking and Association of Plasmodium falciparum Mc-2tm With the Maurer’s Clefts

Authors

Raghavendra Yadavalli, Cleveland State University
John W. Peterson, Cleveland Clinic Foundation
Judith A. Drazba, Cleveland Clinic Foundation
Tobili Y. Sam-Yellowe, Cleveland State UniversityFollow

Document Type

Article

Publication Date

4-1-2021

Publication Title

Pathogens

Disciplines

Biology

Abstract

In this study, we investigated stage specific expression, trafficking, solubility and topology of endogenous PfMC-2TM in P. falciparum (3D7) infected erythrocytes. Following Brefeldin A (BFA) treatment of parasites, PfMC-2TM traffic was evaluated using immunofluorescence with antibodies reactive with PfMC-2TM. PfMC-2TM is sensitive to BFA treatment and permeabilization of infected erythrocytes with streptolysin O (SLO) and saponin, showed that the N and C-termini of PfMC-2TM are exposed to the erythrocyte cytoplasm with the central portion of the protein protected in the MC membranes. PfMC-2TM was expressed as early as 4 h post invasion (hpi), was tightly colocalized with REX-1 and trafficked to the erythrocyte membrane without a change in solubility. PfMC-2TM associated with the MC and infected erythrocyte membrane and was resistant to extraction with alkaline sodium carbonate, suggestive of protein-lipid interactions with membranes of the MC and erythrocyte. PfMC-2TM is an additional marker of the nascent MCs.

DOI

10.3390/pathogens10040431

Version

Publisher's PDF

Recommended Citation

Yadavalli, Raghavendra; Peterson, John W.; Drazba, Judith A.; and Sam-Yellowe, Tobili Y., "Trafficking and Association of Plasmodium falciparum Mc-2tm With the Maurer’s Clefts" (2021). Biological, Geological, and Environmental Faculty Publications. 244.
https://engagedscholarship.csuohio.edu/scibges_facpub/244

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Volume

10

Issue

4