Document Type
Article
Publication Date
2022
Publication Title
PLOS One
Abstract
Disrupting the formation of the oncogenic YAP/TAZ-TEAD transcriptional complex holds substantial therapeutic potential. However, the three protein interaction interfaces of this complex cannot be easily disrupted using small molecules. Here, we report that the pharmacologically active small molecule aurintricarboxylic acid (ATA) acts as a disruptor of the TAZ-TEAD complex. ATA was identified in a high-throughput screen using a TAZ-TEAD AlphaLISA assay that was tailored to identify disruptors of this transcriptional complex. We further used fluorescence polarization assays both to confirm disruption of the TAZ-TEAD complex and to demonstrate that ATA binds to interface 3. We have previously shown that cell-based models that express the oncogenic TAZ-CAMTA1 (TC) fusion protein display enhanced TEAD transcriptional activity because TC functions as an activated form of TAZ. Utilizing cell-based studies and our TC model system, we performed TC/TEAD reporter, RNA-Seq, and qPCR assays and found that ATA inhibits TC/TEAD transcriptional activity. Further, disruption of TC/TEAD and TAZ/TEAD interaction by ATA abrogated anchorage-independent growth, the phenotype most closely linked to dysregulated TAZ/TEAD activity. Therefore, this study demonstrates that ATA is a novel small molecule that has the ability to disrupt the undruggable TAZ-TEAD interface.
DOI
10.1371/journal.pone.0266143
Version
Publisher's PDF
Recommended Citation
Che, Kepeng; Pobbati, Ajaybabu V.; Seavey, Caleb N.; Fedorov, Yuriy; Komar, Anton A.; Burtscher, Ashley; Ma, Shuang; and Rubin, Brian P., "Aurintricarboxylic Acid is a Canonical Disruptor of the TAZ-TEAD Transcriptional Complex" (2022). Biological, Geological, and Environmental Faculty Publications. 268.
https://engagedscholarship.csuohio.edu/scibges_facpub/268
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Volume
17
Issue
4
