Document Type
Article
Publication Date
1-2001
Publication Title
Journal of Evolutionary Biology
Disciplines
Ecology and Evolutionary Biology
Abstract
To examine how the duration of laboratory domestication may affect Drosophila stocks used in studies of thermotolerance, we measured expression of the inducible heat-shock protein Hsp70 and survival after heat shock in D. melanogaster strains recently collected from nature and maintained in laboratory culture for up to 50 or more generations. After an initial increase in both Hsp70 expression and thermotolerance immediately after transfer to laboratory medium, both traits remained fairly constant over time and variation among strains persisted through laboratory domestication. Furthermore, variation in heat tolerance and Hsp70 expression did not correlate with the length of time populations evolved in the laboratory. Therefore, while environmental variation likely contributed most to early shifts in strain tolerance and Hsp70 expression, other population parameters, for example genetic drift, inbreeding, and selection likely affected these traits little. As long as populations are maintained with large numbers of individuals, the culture of insects in the laboratory may have little effect on the tolerance of different strains to thermal stress.
DOI
10.1046/j.1420-9101.2001.00256.x
Version
Postprint
Publisher's Statement
This is the accepted version of the following article: Krebs RA, Roberts SP, Bettencourt BR, Feder ME. 2001. Changes in thermotolerance and Hsp70 expression with domestication in drosophila melanogaster. J Evol Biol 14(1):75-82, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1046/j.1420-9101.2001.00256.x/abstract
Recommended Citation
Krebs RA, Roberts SP, Bettencourt BR, Feder ME. 2001. Changes in thermotolerance and Hsp70 expression with domestication in drosophila melanogaster. J Evol Biol 14(1):75-82.
Volume
14
Issue
1
Comments
Research was supported by National Science Foundation grants IBN94–16124 and IBN97–23298 to MEF, and BRB was supported by a HHMI Predoctoral Fellowship.