Document Type
Article
Publication Date
3-2024
Publication Title
Trends in Genetics
Abstract
In the past decade tRNA sequencing (tRNA-seq) has attracted considerable attention as an important tool for the development of novel approaches to quantify highly modified tRNA species and to propel tRNA research aimed at understanding the cellular physiology and disease and development of tRNA-based therapeutics. Many methods are available to quantify tRNA abundance while accounting for modifications and tRNA charging/acylation. Advances in both library preparation methods and bioinformatic workflows have enabled developments in next-generation sequencing (NGS) workflows. Other approaches forgo NGS applications in favor of hybridization-based approaches. In this review we provide a brief comparative overview of various tRNA quantification approaches, focusing on the advantages and disadvantages of these methods, which together facilitate reliable tRNA quantification.
DOI
10.1016/j.tig.2023.11.001
Version
Publisher's PDF
Recommended Citation
Padhiar, Nigam H.; Katneni, Upendra; Komar, Anton A.; Motorin, Yuri; and Kimchi-Sarfaty, Chava, "Advances in Methods for tRNA Sequencing and Quantification" (2024). Biological, Geological, and Environmental Faculty Publications. 282.
https://engagedscholarship.csuohio.edu/scibges_facpub/282
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Volume
40
Issue
3
Comments
This work was supported by funds from FDA CBER operating funds. This work was also supported by National Heart, Lung, and Blood Institute (NHLBI) grant HL151392.