Document Type
Article
Publication Date
8-7-2026
Publication Title
Biomedicines
Disciplines
Biology | Eye Diseases
Abstract
Objective: Retinal capillary dropout, characterized by acellular capillaries or "ghost vessels," is an early pathological sign of diabetic retinopathy (DR) that remains undetectable through standard clinical imaging techniques until visible morphological changes, such as microaneurysms or hemorrhages, occur. This study aims to develop a non-destructive artificial intelligence (AI)-based method using fluorescein angiography (FA) images to detect early-stage, silent retinal capillary dropout.
Methods: We utilized 94 FA images and corresponding destructive retinal capillary density measurements obtained through retinal trypsin digestion from 51 Nile rats. Early capillary dropout was defined as having an acellular capillary density of >= 18 counts per mm2. A DenseNet based deep learning model was trained to classify images into early capillary dropout or normal. A Bayesian framework incorporating diabetes duration was used to enhance model predictions. RNA sequencing was conducted on retinal vasculature to identify molecular markers associated with capillary early dropout.
Results: The AI-based FA imaging model demonstrated an accuracy of 80.85%, sensitivity of 84.21%, specificity of 75.68%, and an AUC of 0.86. Integration of diabetes duration into a Bayesian predictive framework further improved the model's performance (AUC = 0.90). Transcriptomic analysis identified 43 genes significantly upregulated in retinal tissues preceding capillary dropout. Notably, inflammatory markers such as Bcl2a1, Birc5, and Il20rb were among these genes, indicating that inflammation might play a critical role in early DR pathogenesis.
Conclusions: This study demonstrates that AI-enhanced FA imaging can predict silent retinal capillary dropout before conventional clinical signs of DR emerge. Combining AI predictions with diabetes duration data significantly improves diagnostic performance. The identified gene markers further highlight inflammation as a potential driver in early DR, offering novel insights and potential therapeutic targets for preventing DR progression.
DOI
10.3390/biomedicines13081926
Version
Publisher's PDF
Recommended Citation
Peng, Yiyan; Toh, Huishi; Clegg, Dennis; and Jiiang, Peng, "AI-Enhanced Fluorescein Angiography Detection of Diabetes-Induced Silent Retinal Capillary Dropout and RNA-Seq Identification of Pre-Symptomatic Biomarkers" (2026). Biological, Geological, and Environmental Faculty Publications. 297.
https://engagedscholarship.csuohio.edu/scibges_facpub/297
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Volume
13
Issue
8
