Document Type
Article
Publication Date
7-2018
Publication Title
Aging
Disciplines
Biology
Abstract
The regulation of mechanistic target of rapamycin (mTOR) signaling contributes to the metabolic effects of a calorie restriction (CR) diet. We assayed the effect of CR on the activity of mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2) in the liver of mice at six different times across the day. CR effects on mTORC1 and mTORC2 activities were time-of-day dependent. CR induced mTORC1 activity at one time, reduced at two times and has no effect during other times. CR induced mTORC2 activity at one time of the day and has no effects at other times. Circadian clocks are implemented in the regulation of mTOR signaling in mammals and mechanisms of CR. We assayed the effect of CR on mTOR signaling in the liver of mice deficient for circadian transcriptional regulators BMAL1 and CRYs. The CR induced suppression of mTORC1 activity was observed in both clock mutants, while up regulation of mTORC2 was observed in the liver of CRY deficient but not in the liver of BMAL1 deficient mice. Our finding revealed that CR has different time dependent effect on the activity of mTOR complexes 1 and 2 and suggest that circadian clock protein BMAL1 is involved in the up regulation of mTORC2 upon CR in mammals.
DOI
10.18632/aging.101498]
Version
Publisher's PDF
Recommended Citation
Tulsian, Richa; Velingkaar, Nikkhil; and Kondratov, Roman, "Caloric Restriction Effects on Liver mTOR Signaling are Time‐of‐day Dependent" (2018). Biological, Geological, and Environmental Faculty Publications. 90.
https://engagedscholarship.csuohio.edu/scibges_facpub/90
Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.
Volume
10
Issue
7
Comments
This work was supported by NIH grants 1R01AG039547 and funds from GRHD CSU.