Document Type
Article
Publication Date
10-15-2002
Publication Title
Journal of Pharmaceutical and Biomedical Analysis
Abstract
This paper describes a rapid, simple and sensitive analytical method for the quantitative determination of melatonin in human serum by ESI–MS–MS with direct serum sample injection and on-line extraction. The method uses N-acetyltryptamine as the internal standard. It has high specificity and sensitivity for serum melatonin analysis. The internal calibration curve shows a wide linear range from 0.500 to 200 ng/ml with a correlation coefficient, R2>0.999. The limit of quantitation is 0.500 ng/ml and the limit of detection is 0.100 ng/ml with 10-μl sample injection. The recoveries of serum melatonin at three levels are approximately 70%. The intra-assay precision (n=5) is between 0.8 and 2.0% and the inter-assay precision (n=3) is between 1.5 and 5.9% over the calibration range. This method has a total analysis time of less than 9 min. It can be used for the measurement of melatonin in human blood.
Recommended Citation
Yang, Shuming; Zheng, Xiaohui; Xu, Yan; and Zhou, Xiang, "Rapid Determination of Serum Melatonin by ESI–MS–MS with Direct Sample Injection" (2002). Chemistry Faculty Publications. 208.
https://engagedscholarship.csuohio.edu/scichem_facpub/208
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
DOI
10.1016/S0731-7085(02)00387-4
Version
Postprint
Volume
30
Issue
3
