Heterogeneous Enzyme Immunoassay for Maternal Serum Alpha Fetoprotein by Flow Injection Amperometric Detection of 4-Aminophenol

Document Type

Article

Publication Date

1990

Publication Title

Clinical Chemistry

Abstract

A sandwich-type heterogeneous enzyme immunoassay with flow-injection analysis for alpha-fetoprotein (AFP) in human serum has been developed. 4-Aminophenol, the product of enzymatic reaction, is detected amperometrically. The interassay CV for this electrochemical enzyme immunoassay was <8.2%, with a minimum detection limit for AFP of 0.163 μg/L. The calibration curve had a linear range of 0.316-100 μg/L. Studies with 48 human maternal serum samples, comparing results by this method with those by a commercial kit, showed a good correlation (r=0.961). This procedure provides an alternative method for determining low concentrations of AFP in human maternal serum.

Comments

This work was supported by NSF grant CHE 8217045.

Volume

36

Issue

11

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