Title

Heterogeneous Enzyme Immunoassay for Maternal Serum Alpha Fetoprotein by Flow Injection Amperometric Detection of 4-Aminophenol

Document Type

Article

Publication Date

1990

Publication Title

Clinical Chemistry

Abstract

A sandwich-type heterogeneous enzyme immunoassay with flow-injection analysis for alpha-fetoprotein (AFP) in human serum has been developed. 4-Aminophenol, the product of enzymatic reaction, is detected amperometrically. The interassay CV for this electrochemical enzyme immunoassay was <8.2%, with a minimum detection limit for AFP of 0.163 μg/L. The calibration curve had a linear range of 0.316-100 μg/L. Studies with 48 human maternal serum samples, comparing results by this method with those by a commercial kit, showed a good correlation (r=0.961). This procedure provides an alternative method for determining low concentrations of AFP in human maternal serum.

Comments

This work was supported by NSF grant CHE 8217045.

Volume

36

Issue

11

Share

COinS