Isolation and Quantitation of Plasma Lysophosphatidic Acids by Solid-Phase Extraction and Capillary Electrophoresis

Document Type

Article

Publication Date

2002

Publication Title

Journal of Liquid Chromatography & Related Technologies

Abstract

In this study, a simple and rapid method was developed for isolation and quantitation of lysophosphatidic acid (LPA) molecular species in human plasma. LPA-spiked human plasma (no detectable LPAs) was first isolated and concentrated by Dual-Zone™ C8 solid-phase extraction cartridge. The extracts were then determined by capillary electrophoresis (CE) with indirect UV detection using adenosine monophosphate (AMP) as the UV-absorbing electrolyte. The separation of LPAs molecular species (myristoyl-, palmitoyl-, stearoyl-, and oleoyl-) was achieved within 13 min. With LPA(D) as internal standard in plasma, the method had linear calibration ranges for LPAs from 0.68 μM to 66 μM, and the correlation coefficients were greater than 0.998. This method also had absolute recoveries of LPAs ≥ 83% and reproducibilities of area ratios LPA/I.S. (%CV) ≤ 7% for plasma samples.

DOI

10.1081/JLC-120003263

Volume

25

Issue

6

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