Document Type

Article

Publication Date

4-15-2010

Publication Title

Biosensors and Bioelectronics

Abstract

We investigated in this paper the sensing performance of inherently conductive polymer, poly(3,4-ethylenedioxythiophene) (PEDOT), functionalized with hemin (iron protoporphyrin) as an electrocatalytic reporter. The sensing platform is prepared by electrodeposition of a composite film of hemin–PEDOT on a 30-μm diameter carbon fiber electrode (CFE). The polymerized films were characterized by field emission scanning electron microscopy (FESEM), which pointed to nanostructured films with tortuous pores. The electrocatalytic oxidation of peroxynitrite was characterized by cyclic voltammetry as well as other electrochemical methods. The catalytic current is proportional to the analyte's concentration. Optimized hemin–PEDOT modified CFEs were utilized for the first time to detect ONO2-, with a response time down to 5 s and a limit of detection as low as 200 nM as evidenced by amperometry. Our hemin–PEDOT modified CFEs have a sensitivity of 13 nA/μM, ca.130 times higher than the bare CFE. More work is underway using other metalloporphyrins as electrocalalysts to improve the detection limit, the selectivity, and to further miniaturize these hemin–PEDOT modified electrodes.

Comments

This research was supported by the National Science Foundation (MB; Grant CHE-0848820) and by an FRD and seed grants from CSU-Ohio Board of Regents.

DOI

10.1016/j.bios.2010.01.008

Version

Postprint

Volume

25

Issue

8

Included in

Chemistry Commons

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