Document Type
Article
Publication Date
3-1-2012
Publication Title
Analytical and Bioanalytical Chemistry
Abstract
The antibody specific for the malaria protein, Rhop-3, and FL-Rhop-3, were immobilized on the surface of a gold electrode modified with cysteamine. Colloidal gold was used to enhance the detection signal for Rhop-3 antigens. The Rhop-3 antibody was also immobilized on gold electrodes preactivated with dithiobis(succinimidyl proprionate) (DSP). Immobilization was performed at room temperature and at 37 °C. Cyclic voltammetry (CV) was used to monitor the interaction between the immobilized antibody and its cognate antigen in solution, using ferricyanide, K3Fe(CN)6, as reporting electroactive probe. Tests indicate recognition of Rhop-3 protein by the immobilized antibody. Antigen recognition was enhanced by incubation at 37 °C compared with room-temperature incubation. Our results suggest that an immunosensor can be developed and optimized to aid detection of Rhop-3 antigens in samples from malaria patients. As far as we are aware, this is the first amperometric immunosensor targeting Rhop-3 antigen as a malaria biomarker.
Recommended Citation
Saleh, Salaam; Molek, Susan Moreno; Indika, Perera N.; Riga, Alan; Yellowe, Tobili Sam; and Bayachou, Mekki, "Probe Functionalization With A Rhop-3 Antibody: Toward A Rhop-3 Antigen Immunosensor For Detection of Malaria" (2012). Chemistry Faculty Publications. 325.
https://engagedscholarship.csuohio.edu/scichem_facpub/325
DOI
10.1007/s00216-012-5735-y
Version
Postprint
Publisher's Statement
The final publication is available at Springer via http://dx.doi.org/10.1007/s00216-012-5735-y
Volume
402
Issue
7
Comments
Research funding was provided by Cleveland State University’s Undergraduate Summer Research Program 2010 to both MB and TSY. Other support from an FRD grant (CSU-Ohio Board of Regents) and NSF (grant CHE-0848820) to MB is also recognized.