Intracellular Nitric Oxide Measurement in Human Sperm Using 4, 5-DiaminoFluorescein-2-Diacetate and Flow Cytometry

Authors

R. Sharma, Cleveland State University
S. Bani Hani, Cleveland State University
Mekki Bayachou, Cleveland State UniversityFollow
E. Sabanegh, Cleveland State University
A. Agarwal, Cleveland State UniversityFollow

Document Type

Poster

Publication Date

9-1-2011

Publication Title

Fertility and Sterility

Abstract

Objective: Nitric oxide (NO) has a major contribution in male reproduction, especially in sperm motility. Methods for direct measurement of NO in single cell such as spermatozoa are complex, lack sensitivity, and are non-specific. The objective of this study was to standardize the measurement of intracellular nitric oxide in human spermatozoa using NO specific probe 4,5-diaminofluorescein-2-diacetate (DAF-2DA) and flow cytometry.

Recommended Citation

Sharma, R.; Hani, S. Bani; Bayachou, Mekki; Sabanegh, E.; and Agarwal, A., "Intracellular Nitric Oxide Measurement in Human Sperm Using 4, 5-DiaminoFluorescein-2-Diacetate and Flow Cytometry" (2011). Chemistry Faculty Publications. 349.
https://engagedscholarship.csuohio.edu/scichem_facpub/349

DOI

10.1016/j.fertnstert.2011.07.892

Volume

96

Issue

3