Intracellular Nitric Oxide Measurement in Human Sperm Using 4, 5-DiaminoFluorescein-2-Diacetate and Flow Cytometry
Fertility and Sterility
Objective: Nitric oxide (NO) has a major contribution in male reproduction, especially in sperm motility. Methods for direct measurement of NO in single cell such as spermatozoa are complex, lack sensitivity, and are non-specific. The objective of this study was to standardize the measurement of intracellular nitric oxide in human spermatozoa using NO specific probe 4,5-diaminofluorescein-2-diacetate (DAF-2DA) and flow cytometry.
Sharma, R.; Hani, S. Bani; Bayachou, Mekki; Sabanegh, E.; and Agarwal, A., "Intracellular Nitric Oxide Measurement in Human Sperm Using 4, 5-DiaminoFluorescein-2-Diacetate and Flow Cytometry" (2011). Chemistry Faculty Publications. 349.