Document Type
Article
Publication Date
5-1-2015
Publication Title
Biomedical Chromatography
Abstract
CSUOH0901, a novel anticancer derivative of nimesulide, exhibits very promising anticancer activities in various cancer cell lines. In order to support further pharmacological and toxicological studies of this promising anticancer drug candidate, an LC-MS/MS method was developed and validated in accordance with the US Food and Drug Administration guidelines. The drug molecules were extracted from plasma samples by protein precipitation and then analyzed with LC-ESI-MS/MS. An excellent analyte separation was achieved using a phenomenex C18 column with a mobile phase of 90% methanol and 5 m m of ammonium formate. The validated linear dynamic range was between 0.5 and 100 ng/mL and the achieved correlation coefficient (r2) was >0.9996. The results of inter- and intra-day precision and accuracy were satisfactory, that is, <12% for accuracy and within ±5% for precision at a low and high quality control concentrations, respectively. In addition, the analyte and internal standard (JCC76) were found to be stable under the storage conditions at −20°C for about 2 months. Hence, the acquired results proved that the LC-ESI-MS/MS method developed is precise, accurate and selective for the quantification of CSUOH0901 in plasma, and can be used for pharmacokinetic studies.
Recommended Citation
Voggu, Ramakrishna R.; Alagandula, Ravali; Zhou, Xiang; Su, Bin; Zhong, Bo; and Guo, Baochuan, "A Rapid LC-MS/MS Method for Quantification of CSUOH0901, A Novel Antitumor Agent, in Rat Plasma" (2015). Chemistry Faculty Publications. 376.
https://engagedscholarship.csuohio.edu/scichem_facpub/376
DOI
10.1002/bmc.3365
Version
Postprint
Publisher's Statement
This is the accepted version of the following article: Voggu, R. R.; Alagandula, R.; Zhou, X.; Su, B.; Zhong, B.; Guo, B. A rapid LC-MS/MS method for quantification of CSUOH0901, a novel antitumor agent, in rat plasma. Biomedical Chromatography 2015, 29, 797-802., which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/bmc.3365/full
Volume
29
Issue
5
Comments
We thank Cleveland State University for providing the financial support of this research. We also acknowledge The National Science Foundation Major Research Instrumentation Grant (CHE-0923398), which supported the use of the AB Sciex QTrap 5500 mass spectrometer instrument in the current project.