Document Type
Article
Publication Date
1-1-2008
Publication Title
Steroids
Abstract
Previous studies have demonstrated that cyclooxygenase-2 (COX-2) inhibitor NS-398 decrease aromatase activity at the transcript level in breast cancer cells. However, N-Methyl NS-398, which does not have COX-2 inhibitory activity but has very similar structure to NS-398, decreases aromatase activity and transcription in MCF-7 and MDA-MB-231 breast cells to the same extent as NS-398. This suggests that NS-398 decrease aromatase expression in breast cancer cells via other mechanism(s). Further investigations find that both compounds only decrease aromatase activity stimulated by forskolin/phorbol ester at the transcript level in both breast cancer cell lines and in breast stromal cells from patients. They do not affect aromatase expression and activity stimulated by dexamethasone. Both compounds also suppress MCF-7 cell proliferation stimulated by testosterone. Aromatase inhibition studies using placental microsomes demonstrate that the compounds show only weak direct aromatase inhibition. These results suggest that NS-398 and its N-methyl analog suppress aromatase expression and activity with multiple mechanisms.
Recommended Citation
Su, Bin; Diaz-Cruz, Edgar S.; Landini, Serena; and Brueggemeier, Robert W., "Suppression of Aromatase in Human Breast Cells by A Cyclooxygenase-2 Inhibitor and Its Analog Involves Multiple Mechanisms Independent of Cyclooxygenase-2 Inhibition" (2008). Chemistry Faculty Publications. 411.
https://engagedscholarship.csuohio.edu/scichem_facpub/411
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
DOI
10.1016/j.steroids.2007.09.011
Version
Postprint
Volume
73
Issue
1
Comments
This work was supported by the National Institutes of Health (NIH) Grant R01 CA73698 (to R.W.B.), the NIH Chemistry and Biology Interface Training Program Grant T32 GM08512 (to E.S.D.-C.), and The Ohio State University Comprehensive Cancer Center Breast Cancer Research Fund.